15 Easy Step How-To – Subcutaneous Injection

There are many substances that require a subcutaneous injection rather than the traditional oral or sublingual route of typical nootropics. A major category of these are peptides which often can’t even be taken nasally. Many people are rightfully leery of injecting a substance into their body, and this guide should help make that process a little less scary.

15 Easy Steps to Subcutaneous Injections

You will be using very small insulin needles and it is not going to be nearly as bad as you think. Even if you are squeamish giving blood or find the process painful, you will almost certainly be able to handle a subcutaneous injection. There is a very mild pinch and very little to no blood at all. You won’t need a band-aid, you probably won’t pass out, and you shouldn’t bruise. You don’t need to find a vein or worry about noticeable marks on your skin.

Substances that require subcutaneous (or subQ) injection: SEMAX, SELANK, PT-141 / Melanotan.

At this point you should have a peptide or other injectable and a vial of bacteriostatic water, which is sterile water with benzyl alcohol added as a preservative. A typical bottle of bacteriostatic water should be able to mix several bottles of peptides. You can buy bacteriostatic water a number of places online, including Ceretropic which sells it for around $5.25 a vial. You can also try your hand at making it yourself if you are brave, see our how-to guide.

You will also need to buy insulin syringes either online or at a pharmacy. In the United States, some jurisdictions require a prescription by law and many other retailers will refuse to sell even if it isn’t required by law.

  1. Peptide
  2. Bacteriostatic water
  3. Mixing syringe
  4. Dosing syringes
  5. Alcohol prep pad wipe

I recommend different syringes for mixing and dosing. For mixing, a 100 unit (1cc/ml) 28 gauge is preferable, and for dosing a 50 unit (0.5cc/ml) 31 gauge works well. Depending on what is available at the retailer you may need to adjust slightly.

If a pharmacist refuses to sell you syringes without a prescription and you are not in a place where that is legally required, I recommend telling them that it isn’t for insulin. They will ask what it is for, and if you tell them it is for peptides they will often allow you to purchase. This has worked for me in the past. Basically, dress well, don’t go in too late in the evening, and be honest. Go to the pharmacy, ask for insulin syringes, if they ask about a prescription, tell them no. If they then refuse, tell them it isn’t for insulin, it is for peptides. If you look like you do drugs, have a cleaner cut friend get them for you. Seems to be easier for women to get them than men.

Keep your peptide refrigerated until ready to use. Bacteriostatic water doesn’t need to be refrigerated, although many people do to keep them next to each other and the same temperature.
Once mixed, the peptide will begin to breakdown rapidly. It may only be good for 20-30 days after mixing.
If you have a 10mg substance and you add 1ml of bacteriostatic water, you will be getting a 1mg in 10 units on a U-100 syringe (standard for insulin syringes). This makes dosing very easy. Smaller syringes are usually marked at the 5 unit interval, which would be a 0.5mg dose.


  1. Pop the caps on the water and the peptide.
  2. Wipe the tops of the containers with alcohol wipes every time you go to make an injection.

    Subcutaneous injection into stomach
    Image Source
  3. Take the bacteriostatic water upside down and insert your mixing syringe.
  4. Draw 100 units of water.
  5. Insert syringe into peptide bottle, aiming needle towards side of bottle. You don’t want to shock the peptide by flooding it with water, but instead let the water gently run down the side of the bottle.
  6. When the water is in the peptide bottle, gently swirl the bottle to mix it up. Once again, don’t shake it and damage the peptide.
  7. Withdraw mixing needle, cap and dispose of properly (sharps container!)
  8. Take a fresh dosing syringe (NEVER reuse syringes that have been in a body, dropped on the floor, or compromised in any way) and draw your intended dose from the upside down peptide bottle.
  9. Withdraw and tap the syringe to remove air bubbles like on TV.
  10. Grab the fat of your stomach and pull away from your body. You do not want to do an intramuscular injection, instead you want the subcutaneous fat layer.
  11. Make sure the site is clean, using alcohol prep pad or other cleaning method.
  12. While pinching, insert the needle into the side of the pulled fat.
  13. Slowly inject your beloved chemical!
  14. Withdraw needle, cap, and dispose of properly.
  15. Return peptide to the fridge.

See that wasn’t bad at all! Everyone can subQ!

You can also watch this video below from the Veteran’s Health Administration on how to inject yourself:

Does bacteriostatic water go bad?

One vial of bacteriostatic water should last you through a number of substance mixes. It will come with an expiration date printed on it, but should be good for far longer than that. Most reports say it should last at least two years as long as it is kept out of light which can break down the preservative. Ceretropic recommends using it within 28 days once the seal is broken, but many people keep theirs much longer even opened. As with anything, if you think it has been an long time and might be bad, you might as well throw it out and repurchase since it is fairly inexpensive but the cost of an infection can be great.

Where do I get the bacteriostatic water?

Read our guide on how to make your own bacteriostatic water. If you are squeamish on injecting your own homemade substances, then there are links to purchase online in the guide as well.


Ceretropic.com – Bacteriostatic Water

Image attribution: https://www.flickr.com/photos/feathy123/…

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Working on a better brain for all of us, or die trying. We've been using nootropics for years and have set up this website to help others on their journey for better brain function, memory improvement, energy level increases, fatigue reduction, mood improvement, and more.

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